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Image Search Results
Journal: Nature Communications
Article Title: Spatial exosome analysis using cellulose nanofiber sheets reveals the location heterogeneity of extracellular vesicles
doi: 10.1038/s41467-023-42593-9
Figure Lengend Snippet: a Schematic illustrations of the CNF sheet attachment method. CNF sheets were directly attached to organ surfaces, where they absorbed ascites EVs. After detachment from the organ surface, the sheets were completely dried out, and the cellulose nanofibers were aggregated. CNF sheets were washed, and cellulose nanofibers were opened. Finally, the EVs were released from the CNF sheets. b Representative photos of CNF sheet attachment to the peroneal wall. c , d The size distribution and concentration obtained by nanoparticle tracking assays (NTAs) for mouse ascites EVs by using CNF sheets. n = 10. e Cryo-EM image of EVs recovered from CNF sheet EVs from mouse ascites. f Single-particle quantification was performed using the ExoView platform. n = 3 from independent mice. The numbers of detected EVs are displayed in bar charts. g Schematic illustrations of CNF sheet attachment sites at the liver surface or peritoneum. h A heatmap showing 17 differentially expressed miRNAs from CNF sheet EVs based on small RNA-seq. n = 5: EVs from the liver surface and n = 5: EVs from the peritoneum. Adjusted P -values < 0.05, |log2FC | > 1. c , d – f Data are presented as mean values with SD. Each experiment was repeated at least three times ( c , d – f ).
Article Snippet: The isolated EVs were visualized using a
Techniques: Concentration Assay, Cryo-EM Sample Prep, Single Particle, RNA Sequencing Assay
Journal: Nature Communications
Article Title: Spatial exosome analysis using cellulose nanofiber sheets reveals the location heterogeneity of extracellular vesicles
doi: 10.1038/s41467-023-42593-9
Figure Lengend Snippet: a Illustrative photos of CNF sheets attached to ex vivo human cancer tumors. The right 4 panels indicate CNF sheet attachment, and the left panel indicates CNF sheet soaking for ascites. b Cryo-EM image of EVs recovered from CNF sheet EVs on the tumor surface. c A heatmap showing differentially expressed miRNAs from CNF sheet EVs or tissues based on small RNA-seq. n = 3 each: tumor surface CNF sheet, ascites CNF sheet and cancer tissue. d PCA mapping of miRNA expression, n = 3 each: tumor surface CNF sheet, ascites CNF sheet cancer tissue, serum_ CNF and urine CNF sheet. e Dot plot showing the correlation of miRNA expression in tumor surface CNF sheets vs. cancer tissue and ascites CNF sheets and cancer tissue. f , g Schematic illustrations of the location of samples and bar charts represent relative miRNA expression. n = 3 from independent locations. Data are presented as mean values with SD. The values in the left graph were normalized to tissue miRNA expression, and those in the right graph were normalized to ascites EVs. n = 3 ( h ) PCA mapping and illustrative photos from patient 2 focusing on tumor fluid profiles. i PCA mapping and illustrative photos from patient 2 focusing on metastatic tumors in the greater omentum.
Article Snippet: The isolated EVs were visualized using a
Techniques: Ex Vivo, Cryo-EM Sample Prep, RNA Sequencing Assay, Expressing